h4713 mAb is the antibody against pan HLA-Class II molecules.
h4713 mAb kills malignant lymphoma cells in 30 minutes by making a large hole on the surface of lymphoma cells.
On the other hand, it binds to but does not kill healthy human cells.
In this time-lapse video, the process of killing malignant lymphoma cells (Raji cells) by the h4713 mAb is shown.
The area where the h4713 mAb binds to the cells turns green.
The dead cells are stained red by PI staining.

The upper photo shows the transition of lymphoma cells at 90 minutes after the addition of h4713 mAb. 60 minutes after the addition of h4713 mAb, most of the lymphoma cells turn red, which means that the cancer cells are dead.

The lower photo shows the change after the administration of the control antibody. There is no change in lymphoma cells after binding of h4713 mAb.

h4713 mAb creates cross-links between HLA class II molecules on the surface of lymphoma cells (Raji cells) and aggregates HLA class II molecules. This attracts actin filaments, which disrupt the cytoskeleton and create a hole on the cell surface, which leads to cell death. (Time Laps Video)